from startup import * from ijutils import * from ch.psi.pshell.imaging.Overlays import * import ch.psi.pshell.imaging.Pen as Pen import java.awt.Color as Color import random def get_centroid(source): bi = source.getImage() if bi is None: return None op = show_panel(bi, "Original") ip = load_image(bi) plot(get_histogram(ip), title = "Histogram") grayscale(ip) invert(ip) gaussian_blur(ip) auto_threshold(ip) #binary_erode(ip) show_panel(ip.getBufferedImage(), "Image") (results,output_img)=analyse_particles(ip, 2000,20000, exclude_edges=False, print_table=True) op.clearOverlays() show_panel(output_img.getBufferedImage(), "Outlines") if results.size()>0: centroid = (results.getValue("XM",0), results.getValue("YM",0)) ov = Crosshairs(Pen(Color.ORANGE), java.awt.Point(int(centroid[0]),int(centroid[1])), java.awt.Dimension(15,15)) op.addOverlay(ov) return centroid import ch.psi.pshell.imaging.Filter as Filter class SimulatedSource(Filter): def process(self, img, data): self.img=img if img is None: return None ip = load_image(img) pad_h = int((random.random()-0.5) * 500) pad_v = int((random.random()-0.5) * 500) #print "Pad = " , (pad_h, pad_v) ip = pad_image(ip, -pad_h, pad_h, pad_v, -pad_v, fill_color = Color.BLACK) return ip.getBufferedImage() #return img def waitNext(self, timeout): self.pushImage(self.process(self.img, None)) class ImageStats(DeviceBase): def __init__(self, name, source): DeviceBase.__init__(self, name) self.source = source self.com_x_samples, self.com_y_samples = [], [] class ComX(Readable): def read(self): if len(self.image_stats.com_x_samples)==0: return None return mean(self.image_stats.com_x_samples) self.com_x_mean = ComX(); self.com_x_mean.image_stats = self class ComY(Readable): def read(self): if len(self.image_stats.com_y_samples)==0: return None return mean(self.image_stats.com_y_samples) self.com_y_mean = ComY(); self.com_y_mean.image_stats = self class ComXVar(Readable): def read(self): if len(self.image_stats.com_x_samples)==0: return None return stdev(self.image_stats.com_x_samples) self.com_x_stdev = ComXVar(); self.com_x_stdev.image_stats = self class ComXVar(Readable): def read(self): if len(self.image_stats.com_y_samples)==0: return None return stdev(self.image_stats.com_y_samples) self.com_y_stdev = ComXVar(); self.com_y_stdev.image_stats = self set_device_alias(self.com_x_mean, name + " com x mean") set_device_alias(self.com_y_mean, name + " com y mean") set_device_alias(self.com_x_stdev, name + " com x stdev") set_device_alias(self.com_y_stdev, name + " com y stdev") self.bg_en = False self.num_images = 1 self.initialize() def doUpdate(self): self.com_x_samples, self.com_y_samples = [], [] for i in range(self.num_images): if type(self.source) is not ch.psi.pshell.imaging.FileSource: self.source.waitNext(3000) centroid = get_centroid(self.source) print "cent ", centroid if centroid is not None: self.com_x_samples.append(centroid[0]) self.com_y_samples.append(centroid[1]) def setNumberOfImages(self, value): self.num_images = value def enableBackground(self, value): self.bg_en = value def captureBackground(self, images): self.doInitialize() def doClose(self): pass def start(self): pass def stop(self): pass def get_simulated_source(img): simulated_source = SimulatedSource() simulated_source.img=None simulated_source.initialize() img.addListener(simulated_source) show_panel(simulated_source) return simulated_source if __name__ == "__builtin__": simulated_source = get_simulated_source(image) print get_centroid(simulated_source) add_device(ImageStats("image_stats", simulated_source), True) image_stats.enableBackground(False) for i in range (10): image_stats.update() print image_stats.take(), image_stats.com_x_mean.read(), image_stats.com_y_mean.read() time.sleep(1)