script/test/TstImgSim2.py

@@ -0,0 +1,44 @@
+from ijutils import Roi
+#from sim import *
+import traceback
+run ("imaging/sim")
+
+roi=Roi(0,0,128,128)
+
+"""
+stack = load_test_stack(show=True, size=2)
+ipr, ipi = complex_edge_filtering(stack, show=False) 
+shifts = calculate_shifts(ipr, ipi, roi,  java_code=True)
+#shifts= load_shifts("{images}/TestObjAligner/shifts.mat")
+#stack = load_test_stack(show=True)
+r=translate(stack, shifts, show=True)
+"""
+
+
+
+
+ref = "{images}/TestObjAligner/i210517_040#001.tif"
+img = "{images}/TestObjAligner/i210517_041#001.tif"
+
+ref = open_image(ref)
+img = open_image(img)
+#ref=ref.getBufferedImage()
+#img=img.getBufferedImage()
+#ref=ca.data
+img=grayscale(to_ip(ca.data))
+ref = grayscale(ref.crop([Roi(0,0,452,452)])[0])
+
+def calculate_shift(ref,img, roi, upscale_factor=100, reference_slide=1):
+    ref = to_ip(ref)
+    img = to_ip(img)      
+    stack = create_stack([ref,img])
+    stack.show()
+    ipr, ipi = complex_edge_filtering(stack, show=False) 
+    shifts = calculate_shifts(ipr, ipi, roi,  java_code=True)
+    xoff, yoff =  shifts[1][3], shifts[1][2]
+    error, diffphase =  shifts[1][4], shifts[1][5]
+    r=translate(stack, shifts, show=True)
+    return xoff, yoff,error, diffphase
+
+    
+print calculate_shift(ref,img, roi)