bliven_s
606f3cacaa
Installation is highly manual. This documents the MotionCor2 installation procedure and automates shared file installation. Adds 1.2.6 and 1.3.0 variants.
29 lines
1.6 KiB
Plaintext
29 lines
1.6 KiB
Plaintext
#%Module1.0
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module-whatis "Anisotropic Correction of Beam-induced Motion for Improved Single-particle Electron Cryo-microscopy"
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module-url "http://msg.ucsf.edu/em/software/motioncor2.html"
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module-license "This software may only be downloaded and used for free by academic and/or non-profit users"
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module-maintainer "Spencer Bliven <spencer.bliven@psi.ch>"
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module-help "
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Correction of electron beam-induced sample motion is one of the major factors
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contributing to the recent resolution breakthroughs in cryo-electron
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microscopy. Based on observations that the electron beam induces doming of the
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thin vitreous ice layer, we developed an algorithm to correct anisotropic image
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motion at the single pixel level across the whole frame, suitable for both
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single particle and tomographic images. Iterative, patch-based motion detection
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is combined with spatial and temporal constraints and dose weighting. The
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multi-GPU accelerated program, MotionCor2, is sufficiently fast to keep up with
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automated data collection. The result is an exceptionally robust strategy that
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can work on a wide range of data sets, including those very close to focus or
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with very short integration times, obviating the need for particle polishing.
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Application significantly improves Thon ring quality and 3D reconstruction
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resolution.
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Cite publication: Shawn Q. Zheng, Eugene Palovcak, Jean-Paul Armache, Yifan
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Cheng and David A. Agard (2016) Anisotropic Correction of Beam-induced Motion
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for Improved Single-particle Electron Cryo-microscopy, Nature Methods,
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submitted. BioArxiv: http://biorxiv.org/content/early/2016/07/04/061960
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"
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