#%Module1.0 module-whatis "3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM)" module-url "https://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page" module-license "GPLv2 license" module-maintainer "Spencer Bliven " module-help " RELION (for REgularised LIkelihood OptimisatioN, pronounce rely-on) is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It is developed in the group of Sjors Scheres at the MRC Laboratory of Molecular Biology. Briefly, the ill-posed problem of 3D-reconstruction is regularised by incorporating prior knowledge: the fact that macromolecular structures are smooth, i.e. they have limited power in the Fourier domain. In the corresponding Bayesian framework, many parameters of a statistical model are learned from the data, which leads to objective and high-quality results without the need for user expertise. The underlying theory is given in Scheres (2012) JMB. A more detailed description of its implementation is given in Scheres (2012) JSB. If RELION is useful in your work, please cite at least one of these papers. Scheres(2012) JMB http://www.sciencedirect.com/science/article/pii/S0022283611012290 Scheres(2012) JSB http://www.sciencedirect.com/science/article/pii/S1047847712002481 "