diff --git a/EM/relion/files/variants b/EM/relion/files/variants
index 5782b1f..c7e3efa 100644
--- a/EM/relion/files/variants
+++ b/EM/relion/files/variants
@@ -12,3 +12,5 @@ relion/3.1.2	stable	gcc/9.2.0 openmpi/4.0.5_slurm cuda/11.0.3 b:cmake/3.15.5 b:t
 relion/3.1.3	stable	gcc/9.2.0 openmpi/4.0.5_slurm cuda/11.0.3 b:cmake/3.15.5 b:tiff/4.0.9 b:git/2.22.0
 relion/4.0-beta	deprecated	gcc/9.2.0 openmpi/4.0.5_slurm cuda/11.0.3 b:cmake/3.15.5 b:tiff/4.0.9 b:git/2.22.0
 relion/4.0.0	unstable	gcc/11.2.0 openmpi/4.1.3_slurm cuda/11.5.1 b:cmake/3.23.2 b:tiff/4.3.0 b:git/2.37.0
+relion/4.0.1	unstable	gcc/10.4.0 openmpi/4.1.4_slurm cuda/11.5.1 b:cmake/3.23.2 b:tiff/4.3.0 b:git/2.37.0
+