# Overview ImageJ plugin for reading and writing HDF5 files. For 3D datasets an individual slice can be selected for visualization. Also, especially for very big datasets only every x-th slice can be selected for visualization. This can be done by either specifying a number, e.g. `10` (for the slice 10) or a number with a preceding %, e.g. `%10` (for every 10th image). Indexing starts at 0. # Usage To open a HDF5 file use: ``` File > Import > HDF5... ``` To save to an HDF5 file use: ``` File > SaveAs > HDF5 ``` ## Scripting To use this plugin from the ImageJs' (python) scripting interface these lines can be used to open a dataset: ```python from ch.psi.imagej.hdf5 import HDF5Reader reader = HDF5Reader() stack = reader.open("",False, "/Users/ebner/Desktop/A8_d_400N030_.h5", "/exchange/data_dark", True) ``` # Installation To be able to install this plugin ImageJ need to be run with a Java 7 or greater JVM. * Download latest HDF5 ImageJ plugin from [releases](https://github.com/paulscherrerinstitute/ch.psi.imagej.hdf5/releases). * Go into the ImageJ installation folder and extract the downloaded zip. ``` cd unzip ``` ## Configuration (Optional) If you want to configure the HDF5 Reader as a standard file reader you need to register the reader within the `HandleExtraFileTypes.java` file. This can be done as follows (details on this can be found on: http://albert.rierol.net/imagej_programming_tutorials.html): * Add `HandleExtraFileTypes.java` ```java if (name.endsWith(".h5") || name.endsWith(".hdf5")) { return tryPlugIn("ch.psi.imagej.hdf5.HDF5Reader", path); } ``` * Recompile `HandleExtraFileTypes.java` ``` javac -classpath ij.jar ./plugins/Input-Output/HandleExtraFileTypes.java ``` # Usage ## Mac OS X ``` java -Djava.library.path=./lib/mac64 -Xmx3048m -jar ImageJ64.app/Contents/Resources/Java/ij.jar ``` ## Linux ``` java -Djava.library.path=./lib/linux64 -Xmx3048m -jar ij.jar ``` The `Xmx` setting is quite random it depends on how big hdf5 files you are planning to open. # Fiji Fiji currently comes with Java 6 bundled. As the HDF5 Plugin requires Java 7 or higher we have to instruct Fiji to use an alternative Java. This can be done as follows: ``` cd --java-home /usr/lib/jvm/jre-1.7.0-openjdk.x86_64 -Djava.library.path=lib/linux64 ``` Starting with Java 8 just the LD_LIBRARY_PATH variable need to be set. For MacOSX it is export `DYLD_LIBRARY_PATH=lib/mac64/:$DYLD_LIBRARY_PATH`. # Development To be able to run the tests and plugin from within the following arguments need to be passed: ![RunSettings](RunSettings.png) To create an all in one zip file for installation in a ImageJ installation use: `mvn clean compile assembly:assembly` The zip file contains an all in one jar as well as the required native libraries for Windows, Linux and Mac OS X. Note: to be able to build the package you need to have access to the PSI artifact server. Therefore this only works within the PSI networks and if you have a certain maven configuration. An example Maven settings.xml that you can copy to `~/.m2/settings.xml` is located [here](settings.xml). # Acknowledgements This project was inspired by the ImageJ HDF Plugin of Matthias Schlachter Chair of Pattern Recognition and Image Processing, University of Freiburg, Germany ( https://code.google.com/p/imagej-hdf ) . It is a complete rewrite of the code with the focus on efficiency and maintainability