diff --git a/include/aare/RawFile.hpp b/include/aare/RawFile.hpp
index 74e74c3a..c2cc006c 100644
--- a/include/aare/RawFile.hpp
+++ b/include/aare/RawFile.hpp
@@ -172,6 +172,12 @@ class RawFile : public FileInterface {
     Frame get_frame(size_t frame_index, const size_t roi_index = 0);
 
     void open_subfiles(const size_t roi_index);
+
+    /**
+     * @brief set the ROIs in master file.
+     * @param rois vector of ROIs to set in the RawMasterFile.
+     */
+    void set_ROIs(const std::vector<ROI> &rois);
 };
 
 } // namespace aare
diff --git a/include/aare/RawMasterFile.hpp b/include/aare/RawMasterFile.hpp
index 8ddf44e1..0b48d657 100644
--- a/include/aare/RawMasterFile.hpp
+++ b/include/aare/RawMasterFile.hpp
@@ -13,6 +13,8 @@ using json = nlohmann::json;
 
 namespace aare {
 
+class RawFile; // forward declaration
+
 /**
  * @brief Implementation used in RawMasterFile to parse the file name
  */
@@ -175,6 +177,8 @@ class RawMasterFile {
     void parse_json(std::istream &is);
     void parse_raw(std::istream &is);
     void retrieve_geometry();
+
+    friend class RawFile;
 };
 
 } // namespace aare
\ No newline at end of file
diff --git a/python/src/file.hpp b/python/src/file.hpp
index 9cebef5e..9c365b13 100644
--- a/python/src/file.hpp
+++ b/python/src/file.hpp
@@ -190,8 +190,14 @@ void define_file_io_bindings(py::module &m) {
                      "<ROI: xmin: {} xmax: {} ymin: {} ymax: {}>", self.xmin,
                      self.xmax, self.ymin, self.ymax);
              })
-        .def("__iter__", [](const ROI &self) {
-            return py::make_iterator(&self.xmin, &self.ymax + 1); // NOLINT
+        .def("__iter__",
+             [](const ROI &self) {
+                 return py::make_iterator(&self.xmin, &self.ymax + 1); // NOLINT
+             })
+
+        .def("__eq__", [](const ROI &self, const ROI &other) {
+            return self.xmin == other.xmin && self.xmax == other.xmax &&
+                   self.ymin == other.ymin && self.ymax == other.ymax;
         });
 
 #pragma GCC diagnostic pop
diff --git a/python/tests/test_RawFile.py b/python/tests/test_RawFile.py
index bea0636d..fac13225 100644
--- a/python/tests/test_RawFile.py
+++ b/python/tests/test_RawFile.py
@@ -1,6 +1,6 @@
 # SPDX-License-Identifier: MPL-2.0
 import pytest
-from aare import RawFile
+from aare import RawFile, ROI
 import numpy as np
 
 @pytest.mark.withdata
@@ -120,12 +120,19 @@ def test_read_eiger_udp_port_disabled(test_data_path):
         assert(len(frame) == 2)
         assert frame[0].shape == (256, 512)
         assert frame[1].shape == (256, 1024)
+        rois = f.master.rois
+        assert len(rois) == 2
+        assert rois[0] == ROI(512, 1024, 0, 256)
+        assert rois[1] == ROI(0, 1024, 256, 512)
 
     with RawFile(test_data_path / "raw/eiger/quad_eiger_disabled_bottom_port_master_0.json") as f:
         _, frame = f.read_frame() 
 
         assert frame.shape == (256, 512)
         assert(f.master.disabled_udp_ports == [1])
+        rois = f.master.rois
+        assert len(rois) == 1
+        assert rois[0] == ROI(0, 512, 256, 512)
 
     with RawFile(test_data_path / "raw/eiger/2_modules_eiger_disabled_udp_port_master_0.json") as f:
         _, frame = f.read_rois() 
@@ -134,3 +141,8 @@ def test_read_eiger_udp_port_disabled(test_data_path):
         assert frame[0].shape == (512, 512)
         assert frame[1].shape == (512, 512)
         assert (f.master.disabled_udp_ports == [1, 3, 5, 7])
+
+        rois = f.master.rois
+        assert len(rois) == 2
+        assert rois[0] == ROI(0, 512, 0, 512)
+        assert rois[1] == ROI(1024, 1536, 0, 512)
\ No newline at end of file
diff --git a/src/RawFile.cpp b/src/RawFile.cpp
index a88a289a..bd556adf 100644
--- a/src/RawFile.cpp
+++ b/src/RawFile.cpp
@@ -210,6 +210,8 @@ RawFile::RawFile(const std::filesystem::path &fname, const std::string &mode)
             std::vector<ROI> rois = get_rois_from_disabled_udp_ports(
                 disabled_ports, udp_port_types, m_geometry);
 
+            set_ROIs(rois);
+
             m_subfiles.resize(rois.size());
 
             m_ROI_geometries.reserve(rois.size());
@@ -689,4 +691,6 @@ size_t RawFile::frame_number(size_t frame_index) {
     return m_subfiles[0][0]->frame_number(frame_index);
 }
 
+void RawFile::set_ROIs(const std::vector<ROI> &rois) { m_master.m_rois = rois; }
+
 } // namespace aare
diff --git a/src/RawFile.test.cpp b/src/RawFile.test.cpp
index e2d27315..e922dae2 100644
--- a/src/RawFile.test.cpp
+++ b/src/RawFile.test.cpp
@@ -421,6 +421,11 @@ TEST_CASE("Read Jungfrau frame with disabled UDP ports",
         auto frame = f.read_frame();
         REQUIRE(frame.cols() == 1024);
         REQUIRE(frame.rows() == 256);
+
+        auto rois = f.master().rois();
+
+        REQUIRE(rois.value().size() == 1);
+        REQUIRE(rois.value()[0] == ROI{0, 1024, 0, 256});
     }
 
     SECTION("disabled bottom port") {
@@ -435,6 +440,9 @@ TEST_CASE("Read Jungfrau frame with disabled UDP ports",
         auto frame = f.read_frame();
         REQUIRE(frame.cols() == 1024);
         REQUIRE(frame.rows() == 256);
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 1);
+        REQUIRE(rois.value()[0] == ROI{0, 1024, 256, 512});
     }
     SECTION("2 modules - top ports disabled") {
         auto fpath = test_data_path() / "raw/jungfrau" /
@@ -459,6 +467,10 @@ TEST_CASE("Read Jungfrau frame with disabled UDP ports",
         REQUIRE(frame[0].rows() == 256);
         REQUIRE(frame[1].cols() == 1024);
         REQUIRE(frame[1].rows() == 256);
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 2);
+        REQUIRE(rois.value()[0] == ROI{0, 1024, 0, 256});
+        REQUIRE(rois.value()[1] == ROI{0, 1024, 512, 768});
     }
     SECTION("2 modules - top ports disabled - bottom port disabled") {
         auto fpath = test_data_path() / "raw/jungfrau" /
@@ -472,6 +484,9 @@ TEST_CASE("Read Jungfrau frame with disabled UDP ports",
         auto frame = f.read_frame();
         REQUIRE(frame.cols() == 1024);
         REQUIRE(frame.rows() == 512);
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 1);
+        REQUIRE(rois.value()[0] == ROI{0, 1024, 256, 768});
     }
     SECTION("4 modules- mixed ports disabled") {
         auto fpath = test_data_path() / "raw/jungfrau" /
@@ -497,6 +512,12 @@ TEST_CASE("Read Jungfrau frame with disabled UDP ports",
         REQUIRE(frames[2].rows() == 256);
         REQUIRE(frames[3].cols() == 1024);
         REQUIRE(frames[3].rows() == 256);
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 4);
+        REQUIRE(rois.value()[0] == ROI{0, 1024, 0, 256});
+        REQUIRE(rois.value()[1] == ROI{0, 1024, 512, 768});
+        REQUIRE(rois.value()[2] == ROI{1024, 2048, 256, 512});
+        REQUIRE(rois.value()[3] == ROI{1024, 2048, 768, 1024});
     }
 }
 
@@ -514,6 +535,10 @@ TEST_CASE("Read Moench frame with disabled UDP ports",
         auto frame = f.read_frame();
         REQUIRE(frame.cols() == 400);
         REQUIRE(frame.rows() == 200);
+        auto rois = f.master().rois();
+
+        REQUIRE(rois.value().size() == 1);
+        REQUIRE(rois.value()[0] == ROI{0, 400, 0, 200});
     }
 
     SECTION("disabled bottom port") {
@@ -528,6 +553,10 @@ TEST_CASE("Read Moench frame with disabled UDP ports",
         auto frame = f.read_frame();
         REQUIRE(frame.cols() == 400);
         REQUIRE(frame.rows() == 200);
+        auto rois = f.master().rois();
+
+        REQUIRE(rois.value().size() == 1);
+        REQUIRE(rois.value()[0] == ROI{0, 400, 200, 400});
     }
 }
 
@@ -544,10 +573,12 @@ TEST_CASE("Read Eiger frame with disabled UDP ports",
                 std::vector<std::string>{"left", "right"});
         auto frame = f.read_frame();
 
-        std::cout << fmt::format("num rows {}, num cols {}", frame.rows(),
-                                 frame.cols());
         REQUIRE(frame.cols() == 512);
         REQUIRE(frame.rows() == 512);
+
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 1);
+        REQUIRE(rois.value()[0] == ROI{512, 1024, 0, 512});
     }
     SECTION("disabled right port") {
         auto fpath = test_data_path() / "raw/eiger" /
@@ -561,6 +592,9 @@ TEST_CASE("Read Eiger frame with disabled UDP ports",
         auto frame = f.read_frame();
         REQUIRE(frame.cols() == 512);
         REQUIRE(frame.rows() == 512);
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 1);
+        REQUIRE(rois.value()[0] == ROI{0, 512, 0, 512});
     }
     SECTION("2 full modules stacked vertically - right ports disabled") {
         auto fpath = test_data_path() / "raw/eiger" /
@@ -583,6 +617,10 @@ TEST_CASE("Read Eiger frame with disabled UDP ports",
         REQUIRE(frames[0].rows() == 512);
         REQUIRE(frames[1].cols() == 512);
         REQUIRE(frames[1].rows() == 512);
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 2);
+        REQUIRE(rois.value()[0] == ROI{0, 512, 0, 512});
+        REQUIRE(rois.value()[1] == ROI{1024, 1536, 0, 512});
     }
     SECTION("quad module - bottom port disabled") {
         auto fpath = test_data_path() / "raw/eiger" /
@@ -597,6 +635,9 @@ TEST_CASE("Read Eiger frame with disabled UDP ports",
         auto frame = f.read_frame();
         REQUIRE(frame.cols() == 512);
         REQUIRE(frame.rows() == 256);
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 1);
+        REQUIRE(rois.value()[0] == ROI{0, 512, 256, 512});
     }
     SECTION("only one port disabled") {
         auto fpath = test_data_path() / "raw/eiger" /
@@ -614,5 +655,10 @@ TEST_CASE("Read Eiger frame with disabled UDP ports",
         REQUIRE(frame[0].rows() == 256);
         REQUIRE(frame[1].cols() == 1024);
         REQUIRE(frame[1].rows() == 256);
+        auto rois = f.master().rois();
+        REQUIRE(rois.value().size() == 2);
+
+        REQUIRE(rois.value()[0] == ROI{512, 1024, 0, 256});
+        REQUIRE(rois.value()[1] == ROI{0, 1024, 256, 512});
     }
 }