mirror of
https://github.com/cdtools-developers/cdtools.git
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179 lines
6.6 KiB
Python
179 lines
6.6 KiB
Python
from __future__ import division, print_function, absolute_import
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import numpy as np
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import torch as t
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from matplotlib import pyplot as plt
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import pickle
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import argparse
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from scipy import fftpack
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from CDTools.tools import cmath, plotting
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from CDTools.tools import image_processing as ip
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def standardize(probe, obj, obj_slice=None):
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# First, we normalize the probe intensity to a fixed value.
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# Should this be the maximum or the integrated intensity? I think
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# probably the integrated intensity. We set the average per-[ixel
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# intensity in the probe to be one
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normalization = np.sqrt(np.sum(np.abs(probe)**2) / len(probe.ravel()))
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probe = cmath.complex_to_torch(probe / normalization)
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obj = cmath.complex_to_torch(obj * normalization)
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# Default slice of the object to use for alignment, etc.
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if obj_slice is None:
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obj_slice = np.s_[(obj.shape[0]//8)*3:(obj.shape[0]//8)*5,
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(obj.shape[1]//8)*3:(obj.shape[1]//8)*5]
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# Now we get rid of the probe's phase ramp
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# Currently disabled
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#center_freq = ip.centroid_sq(cmath.fftshift(t.fft(probe,2)),comp=True)
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#center_freq -= (t.tensor(probe.shape[:-1]) // 2).to(t.float32)
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#center_freq /= t.tensor(probe.shape[:-1]).to(t.float32)
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#Is, Js = np.mgrid[:probe.shape[0],:probe.shape[1]]
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#probe_phase_ramp = cmath.expi(2*np.pi *
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# (center_freq[0] * t.tensor(Is).to(t.float32) +
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# center_freq[1] * t.tensor(Js).to(t.float32)))
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#probe = cmath.cmult(probe, cmath.cconj(probe_phase_ramp))
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#Is, Js = np.mgrid[:obj.shape[0],:obj.shape[1]]
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#obj_phase_ramp = cmath.expi(2*np.pi *
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# (center_freq[0] * t.tensor(Is).to(t.float32) +
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# center_freq[1] * t.tensor(Js).to(t.float32)))
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#obj = cmath.cmult(obj, obj_phase_ramp)
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# Then, we set them to consistent absolute phases
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probe_angle = cmath.cphase(t.sum(probe,dim=(0,1)))
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obj_angle = cmath.cphase(t.sum(obj[obj_slice],dim=(0,1)))
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probe = cmath.cmult(probe, cmath.expi(-probe_angle))
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obj = cmath.cmult(obj, cmath.expi(-obj_angle))
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return probe, obj
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def synthesize_reconstructions(probes, objects, use_probe=False, obj_slice=None):
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if obj_slice is None:
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obj_slice = np.s_[(objects[0].shape[0]//8)*3:(objects[0].shape[0]//8)*5,
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(objects[0].shape[1]//8)*3:(objects[0].shape[1]//8)*5]
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synth_probe, synth_obj = standardize(probes[0], objects[0])
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obj_stack = [cmath.torch_to_complex(synth_obj)]
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for i, (probe, obj) in enumerate(zip(probes[1:],objects[1:])):
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probe, obj = standardize(probe, obj)
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probe = probe[0]
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print(i)
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#plt.imshow(np.angle(cmath.torch_to_complex(obj[obj_slice])))
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#plt.show()
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if use_probe:
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shift = ip.find_shift(synth_probe,probe, resolution=50)
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else:
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shift = ip.find_shift(synth_obj[obj_slice],obj[obj_slice], resolution=50)
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obj = ip.sinc_subpixel_shift(obj,np.array(shift))
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probe = ip.sinc_subpixel_shift(probe,tuple(shift))
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#obj = t.roll(obj,tuple(int(s) for s in shift),dims=(0,1))
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#probe = t.roll(probe,tuple(int(s) for s in shift),dims=(0,1))
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synth_probe += probe
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synth_obj += obj
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obj_stack.append(cmath.torch_to_complex(obj))
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# If there only was one image
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try:
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i
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except:
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i = -1
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synth_probe = cmath.torch_to_complex(synth_probe)
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synth_obj = cmath.torch_to_complex(synth_obj)
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return synth_probe/(i+2), synth_obj/(i+2), obj_stack
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def calc_prtf(synth_obj, objects, basis, obj_slice=None):
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if obj_slice is None:
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obj_slice = np.s_[(objects[0].shape[0]//8)*3:(objects[0].shape[0]//8)*5,
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(objects[0].shape[1]//8)*3:(objects[0].shape[1]//8)*5]
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synth_obj = cmath.complex_to_torch(synth_obj[obj_slice])
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synth_fft = cmath.cabssq(cmath.fftshift(t.fft(synth_obj,2))).numpy()
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prtfs = []
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for obj in objects:
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obj = cmath.complex_to_torch(obj[obj_slice])
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single_fft = cmath.cabssq(cmath.fftshift(t.fft(obj,2))).numpy()
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di = np.linalg.norm(basis[:,0])
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dj = np.linalg.norm(basis[:,1])
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i_freqs = fftpack.fftshift(fftpack.fftfreq(single_fft.shape[0],d=di))
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j_freqs = fftpack.fftshift(fftpack.fftfreq(single_fft.shape[1],d=dj))
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Js,Is = np.meshgrid(j_freqs,i_freqs)
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Is = Is - np.mean(Is)
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Js = Js - np.mean(Js)
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Rs = np.sqrt(Is**2+Js**2)
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single_ints, bins = np.histogram(Rs,bins=100,weights=single_fft)
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synth_ints, bins = np.histogram(Rs,bins=100,weights=synth_fft)
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prtfs.append(synth_ints/single_ints)
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return bins[:-1], np.mean(prtfs,axis=0)
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def make_argparser():
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parser = argparse.ArgumentParser(description=__doc__)
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parser.add_argument('file', help='The reconstruction file to calculate metrics for')
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parser.add_argument('--use-probe', '-up', action='store_true', help='Use the probe instead of the object to align the reconstructions')
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return parser
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if __name__ == '__main__':
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args = make_argparser().parse_args()
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with open(args.file, 'rb') as f:
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dataset = pickle.load(f)
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synth_probe, synth_obj, aligned_objs = synthesize_reconstructions(
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dataset['probe'], dataset['obj'], args.use_probe)
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freqs, prtf = calc_prtf(synth_obj, aligned_objs, dataset['basis'])
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print(np.linalg.norm(dataset['basis'],axis=0))
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plotting.plot_phase(dataset['probe'][0][0],basis=1e6*dataset['basis'])
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plotting.plot_amplitude(dataset['probe'][0][0],basis=1e6*dataset['basis'])
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plotting.plot_colorized(dataset['probe'][0][0],basis=1e6*dataset['basis'])
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#plotting.plot_amplitude(synth_obj[400:750,450:850],basis=1e6*dataset['basis'])
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#plotting.plot_colorized(synth_obj[400:750,450:850],basis=1e6*dataset['basis'])
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#plotting.plot_phase(synth_obj[400:750,450:850],basis=1e6*dataset['basis'])
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plotting.plot_amplitude(synth_obj[::-1,::-1][450:900,325:775],basis=1e6*dataset['basis'])
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plotting.plot_phase(synth_obj[::-1,::-1][450:900,325:775],basis=1e6*dataset['basis'])
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plotting.plot_colorized(synth_obj[::-1,::-1][450:900,325:775],basis=1e6*dataset['basis'])
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plt.figure()
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real_translations = dataset['basis'].dot(dataset['translation'][0].transpose())
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real_translations -= np.min(real_translations,axis=1)[:,None]
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plt.plot(real_translations[0]*1e6,real_translations[1]*1e6,'k.')
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plt.plot(real_translations[0]*1e6,real_translations[1]*1e6,'b-',linewidth=0.5)
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plt.figure()
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plt.plot(freqs*1e-6, prtf)
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plt.show()
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