diff --git a/bec_widgets/widgets/plots/image/setting_widgets/image_roi_tree.py b/bec_widgets/widgets/plots/image/setting_widgets/image_roi_tree.py
index 174d2c1d..c7fa5cad 100644
--- a/bec_widgets/widgets/plots/image/setting_widgets/image_roi_tree.py
+++ b/bec_widgets/widgets/plots/image/setting_widgets/image_roi_tree.py
@@ -3,6 +3,7 @@ from __future__ import annotations
 import math
 from typing import TYPE_CHECKING
 
+from bec_lib import bec_logger
 from bec_qthemes import material_icon
 from qtpy.QtCore import QEvent, Qt
 from qtpy.QtGui import QColor
@@ -39,6 +40,9 @@ if TYPE_CHECKING:
     from bec_widgets.widgets.plots.image.image import Image
 
 
+logger = bec_logger.logger
+
+
 class ROILockButton(QToolButton):
     """Keeps its icon and checked state in sync with a single ROI."""
 
@@ -447,6 +451,18 @@ class ROIPropertyTree(BECWidget, QWidget):
     def cleanup(self):
         self.cmap.close()
         self.cmap.deleteLater()
+        if self.controller and hasattr(self.controller, "rois"):
+            for roi in self.controller.rois:  # disconnect all signals from ROIs
+                try:
+                    if isinstance(roi, RectangularROI):
+                        roi.edgesChanged.disconnect()
+                    else:
+                        roi.centerChanged.disconnect()
+                    roi.penChanged.disconnect()
+                    roi.nameChanged.disconnect()
+                except (RuntimeError, TypeError) as e:
+                    logger.error(f"Failed to disconnect roi qt signal: {e}")
+
         super().cleanup()
 
 
diff --git a/tests/unit_tests/test_image_roi_tree.py b/tests/unit_tests/test_image_roi_tree.py
index c0dc38ea..6480e870 100644
--- a/tests/unit_tests/test_image_roi_tree.py
+++ b/tests/unit_tests/test_image_roi_tree.py
@@ -399,3 +399,35 @@ def test_new_roi_respects_global_lock(roi_tree, image_widget, qtbot):
     assert not roi.movable
     # Disable global lock again
     roi_tree.lock_all_action.action.setChecked(False)
+
+
+def test_cleanup_disconnect_signals(roi_tree, image_widget):
+    """Test that cleanup disconnects ROI signals so further changes do not update the tree."""
+    # Add a rectangular ROI
+    roi = image_widget.add_roi(kind="rect", name="cleanup_test", pos=(10, 10), size=(20, 20))
+    item = roi_tree.roi_items[roi]
+
+    # Test that signals are connected before cleanup
+    pre_name = item.text(roi_tree.COL_ROI)
+    pre_coord = item.child(2).text(roi_tree.COL_PROPS)
+    # Change ROI properties to see updates
+    roi.label = "connected_name"
+    roi.setPos(30, 30)
+    # Verify that the tree item updated
+    assert item.text(roi_tree.COL_ROI) == "connected_name"
+    assert item.child(2).text(roi_tree.COL_PROPS) != pre_coord
+
+    # Perform cleanup to disconnect signals
+    roi_tree.cleanup()
+
+    # Store initial state
+    initial_name = item.text(roi_tree.COL_ROI)
+    initial_coord = item.child(2).text(roi_tree.COL_PROPS)
+
+    # Change ROI properties after cleanup
+    roi.label = "changed_name"
+    roi.setPos(50, 50)
+
+    # Verify that the tree item was not updated
+    assert item.text(roi_tree.COL_ROI) == initial_name
+    assert item.child(2).text(roi_tree.COL_PROPS) == initial_coord