195 lines
8.5 KiB
Python
195 lines
8.5 KiB
Python
import sys
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sys.path.append('./src')
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from pathlib import Path
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from omegaconf import OmegaConf
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import torch
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from tqdm import tqdm
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from matplotlib import pyplot as plt
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import numpy as np
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import h5py
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from models import get_double_photon_model_class
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from datasets import doublePhotonInferenceDataset
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torch.manual_seed(42)
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torch.cuda.manual_seed(42)
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np.random.seed(42)
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torch.backends.cudnn.deterministic = True
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torch.backends.cudnn.benchmark = False
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conf = OmegaConf.load("Configs/infer_2photon.yaml")
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NX, NY = conf.data.names[conf.experiment.name].NX, conf.data.names[conf.experiment.name].NY
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def inv0(p): return p
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def inv1(p): return torch.stack([-p[..., 0], p[..., 1]], dim=-1)
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def inv2(p): return torch.stack([p[..., 0], -p[..., 1]], dim=-1)
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def inv3(p): return -p
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def inv4(p): return torch.stack([p[..., 1], p[..., 0]], dim=-1)
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def inv5(p): return torch.stack([p[..., 1], -p[..., 0]], dim=-1)
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def inv6(p): return torch.stack([-p[..., 1], p[..., 0]], dim=-1)
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def inv7(p): return torch.stack([-p[..., 1], -p[..., 0]], dim=-1)
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INVERSE_TRANSFORMS = {
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0: inv0, 1: inv1, 2: inv2, 3: inv3,
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4: inv4, 5: inv5, 6: inv6, 7: inv7,
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}
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def apply_inverse_transforms(predictions: torch.Tensor, numberOfAugOps: int) -> torch.Tensor:
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N = predictions.shape[0] // numberOfAugOps
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preds = predictions.view(N, numberOfAugOps, 2)
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corrected = torch.zeros_like(preds)
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for idx in range(numberOfAugOps):
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corrected[:, idx, :] = INVERSE_TRANSFORMS[idx](preds[:, idx, :])
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return corrected.mean(dim=1)
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def prepare_output_folder(conf):
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if conf.data.normalize:
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normalize_suffix = '_normalized'
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else:
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normalize_suffix = ''
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output_base = Path(conf.experiment.output_base) / conf.experiment.name / conf.model.experiment_name / f'augX{conf.inference.num_aug_ops}{normalize_suffix}'
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output_base.mkdir(parents=True, exist_ok=True)
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OmegaConf.save(conf, output_base / 'config.yaml')
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return output_base
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def get_files_list(conf):
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task_conf = conf.data.names[conf.experiment.name]
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file_pattern = task_conf.file_pattern
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start, end = task_conf.file_range
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files = [str(Path(conf.data.sample_folder) / file_pattern.format(i)) for i in range(start, end)]
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return files
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def run_inference(model, data_loader, conf):
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all_predictions = []
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with torch.no_grad():
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for batch_idx, batch in enumerate(tqdm(data_loader, desc="Inferring")):
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inputs, _ = batch
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inputs = inputs.cuda()
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outputs = model(inputs).view(-1, 2) # 2B x 2
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all_predictions.append(outputs.cpu())
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all_predictions = torch.cat(all_predictions, dim=0)
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# all_predictions = apply_inverse_transforms(all_predictions, conf.inference.num_aug_ops)
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all_predictions += torch.tensor([conf.data.names[conf.experiment.name].nSize/2., conf.data.names[conf.experiment.name].nSize/2.]).unsqueeze(0) # adjust back to original coordinate system
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print(f'mean x = {torch.mean(all_predictions[:, 0])}, std x = {torch.std(all_predictions[:, 0])}')
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print(f'mean y = {torch.mean(all_predictions[:, 1])}, std y = {torch.std(all_predictions[:, 1])}')
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referencePoints = data_loader.dataset.referencePoint ### the lower-left corner of the cluster in absolute coordinate
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referencePoints = np.repeat(referencePoints, 2, axis=0) ### duplicate reference points for 2-photon clusters
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return all_predictions.numpy(), referencePoints
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def accumulate_hits(predictions: np.ndarray, reference_points: np.ndarray,
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binning_factor: int):
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### ret
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ml_super_frame = np.zeros((NY*binning_factor, NX*binning_factor), dtype=np.int32)
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count_frame = np.zeros((NY, NX), dtype=np.int32)
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subpixel_dist = np.zeros((binning_factor, binning_factor), dtype=np.int32)
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### absolute coordinate = predicted subpixel + reference point
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absolute_positions = predictions + reference_points
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hit_x_superpixel_idx = np.floor(absolute_positions[:, 0] * binning_factor).astype(int)
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hit_x_superpixel_idx = np.clip(hit_x_superpixel_idx, 0, NX*binning_factor-1)
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hit_y_superpixel_idx = np.floor(absolute_positions[:, 1] * binning_factor).astype(int)
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hit_y_superpixel_idx = np.clip(hit_y_superpixel_idx, 0, NY*binning_factor-1)
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np.add.at(ml_super_frame, (hit_y_superpixel_idx, hit_x_superpixel_idx), 1)
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hit_x_pixel_idx = np.floor(absolute_positions[:, 0]).astype(int)
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hit_x_pixel_idx = np.clip(hit_x_pixel_idx, 0, NX-1)
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hit_y_pixel_idx = np.floor(absolute_positions[:, 1]).astype(int)
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hit_y_pixel_idx = np.clip(hit_y_pixel_idx, 0, NY-1)
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np.add.at(count_frame, (hit_y_pixel_idx, hit_x_pixel_idx), 1)
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subpixel_x_idx = np.floor((absolute_positions[:, 0] % 1) * binning_factor).astype(int)
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subpixel_y_idx = np.floor((absolute_positions[:, 1] % 1) * binning_factor).astype(int)
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np.add.at(subpixel_dist, (subpixel_y_idx, subpixel_x_idx), 1)
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return ml_super_frame, count_frame, subpixel_dist
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def save_results(ml_super_frame, count_frame, subpixel_dist,
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roi: list, binning_factor: int, output_dir: Path):
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x_st, x_ed, y_st, y_ed = roi
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# 1. super-resolution frame
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plt.figure(figsize=(8, 8))
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plt.imshow(ml_super_frame[y_st*binning_factor:y_ed*binning_factor, x_st*binning_factor:x_ed*binning_factor], origin='lower', extent=[x_st, x_ed, y_st, y_ed])
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plt.colorbar(label='Counts')
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plt.title('ML Super-Resolution Frame')
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plt.xlabel('X (pixel)')
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plt.ylabel('Y (pixel)')
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plt.savefig(output_dir / '2Photon_ML_superFrame.png', dpi=300, bbox_inches='tight')
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plt.clf()
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np.save(output_dir / '2Photon_ML_superFrame.npy', ml_super_frame)
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# 2. count frame
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plt.imshow(count_frame[y_st:y_ed, x_st:x_ed], origin='lower', extent=[x_st, x_ed, y_st, y_ed])
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plt.colorbar(label='Counts')
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plt.title('Photon Count Frame')
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plt.xlabel('X (pixel)')
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plt.ylabel('Y (pixel)')
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plt.savefig(output_dir / '2Photon_count_Frame.png', dpi=300, bbox_inches='tight')
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plt.clf()
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np.save(output_dir / '2Photon_count_Frame.npy', count_frame)
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# 3. subpixel distribution
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plt.imshow(subpixel_dist, origin='lower', extent=[0, 1, 0, 1])
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plt.colorbar(label='Counts')
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plt.title('Subpixel Distribution')
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plt.xlabel('Subpixel X')
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plt.ylabel('Subpixel Y')
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plt.savefig(output_dir / '2Photon_subpixel_Distribution.png', dpi=300, bbox_inches='tight')
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plt.close()
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np.save(output_dir / '2Photon_subpixel_Distribution.npy', subpixel_dist)
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std, mean = np.std(subpixel_dist), np.mean(subpixel_dist)
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print(f"[Plotting]: Sub-pixel distribution: Std/Mean: {std/mean:.4f}")
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print(f"Results saved to: {output_dir}")
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if __name__ == "__main__":
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### output folder preparation
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output_dir = prepare_output_folder(conf)
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### model loading
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model = get_double_photon_model_class(conf.model.version)().cuda()
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model.load_state_dict(torch.load(f'{conf.model.base_dir}/{conf.model.experiment_name}/Models/{conf.model.name}', weights_only=True))
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# model.eval()
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### data loading
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files_list = get_files_list(conf)
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roi = conf.data.names[conf.experiment.name].roi
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BinningFactor = conf.inference.binning_factor
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numberOfAugOps = conf.inference.num_aug_ops
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flag_normalize = conf.data.normalize
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nChunks = np.ceil(len(files_list) / 16).astype(int)
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ml_super_frame = np.zeros((NY*BinningFactor, NX*BinningFactor), dtype=np.int32)
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count_frame = np.zeros((NY, NX), dtype=np.int32)
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subpixel_dist = np.zeros((BinningFactor, BinningFactor), dtype=np.int32)
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for idxChunk in range(nChunks):
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start_idx = idxChunk * conf.inference.chunk_size
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end_idx = min(start_idx + conf.inference.chunk_size, len(files_list))
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chunk_files = files_list[start_idx:end_idx]
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print(f'[Inferring] Chunk {idxChunk+1}/{nChunks}: Loading files {start_idx} to {end_idx}...')
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dataset = doublePhotonInferenceDataset(
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chunk_files,
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sampleRatio=1.0,
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datasetName=f'Inference_Chunk{idxChunk+1}',
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# numberOfAugOps=numberOfAugOps,
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nSize=conf.data.names[conf.experiment.name].nSize,
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)
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dataLoader = torch.utils.data.DataLoader(
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dataset,
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batch_size=8192,
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shuffle=False,
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num_workers=16,
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pin_memory=True,
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)
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predictions, reference_points = run_inference(model, dataLoader, conf)
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ml_super_frame_chunk, count_frame_chunk, subpixel_dist_chunk = accumulate_hits(predictions, reference_points, BinningFactor)
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ml_super_frame += ml_super_frame_chunk
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count_frame += count_frame_chunk
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subpixel_dist += subpixel_dist_chunk
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save_results(ml_super_frame, count_frame, subpixel_dist, roi, BinningFactor, output_dir)
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